|
KMID : 1144820190250020123
|
|
대한의생명과학회지
2019 Volume.25 No. 2 p.123 ~ p.123
|
|
|
Inhibitory Effect of Scopoletin on U46619-induced Platelet Aggregation through Regulation of Ca2+ Mobilization
|
|
Lee Dong-Ha
|
|
|
Abstract
|
|
|
|
Platelet aggregation is essential for hemostatic process in case of blood vessels damages. However, excessive platelet aggregation can cause cardiovascular disorders including atherosclerosis, thrombosis and myocardial infarction. Scopoletin is usually found in the roots of genus Scopolia or Artemisia, and is known to have anticoagulant and anti-malarial effects. This study investigated the effect of scopoletin on human platelet aggregation induced by U46619, an analogue of thromboxane A2 (TXA2). Scopoletin had anti-platelet effects by down-regulating TXA2 and intracellular Ca2+ mobilization ([Ca2+]i), the aggregation-inducing molecules generated in activated platelets. On the other hand, scopoletin increased the levels of cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP), which are known to be intracellular Ca2+ antagonists. This resulted in inhibition of fibrinogen binding to αIIb/β3 in U46619-induced human platelet aggregation. In addition, scopoletin inhibited the release of adenosine trisphosphate (ATP) in dose-dependent manner. This result means that the aggregation amplification activity through the granule secretion in platelets was suppressed by scopoletin. Therefore, we demonstrated that scopoletin has a potent antiplatelet effect and is highly likely to prevent platelet-derived vascular disease.
|
|
|
KEYWORD
|
|
|
Scopoletin, Platelet aggregation, Intracellular Ca2+, Fibrinogen binding
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|